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lightcycler 480 software version 1 5 1 62  (Roche)


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    Structured Review

    Roche lightcycler 480 software version 1 5 1 62
    Lightcycler 480 Software Version 1 5 1 62, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+480+software+version+1+5/LightCycler+480+System/pm41964673-51-32-37
    Average 99 stars, based on 6 article reviews
    lightcycler 480 software version 1 5 1 62 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes.
    Article Snippet: Samples were diluted 1:5 within nuclease-free water (Gibco) and used alongside respective primers and LightCycler 480 SYBR Green I Master Kit (Roche).

    Article Title: 3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV
    Article Snippet: Samples were mixed with molecular biology-grade water (Gibco), primers and LightCycler 480 SYBR Green I Master Kit (Roche).

    Quantitative RT-PCR:

    Article Title: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes
    Article Snippet: Samples were diluted 1:5 within nuclease-free water (Gibco) and used alongside respective primers and LightCycler 480 SYBR Green I Master Kit (Roche). .. RT-qPCR reactions were performed in a LightCycler 480 Instrument II 384-well block (Roche) and quantification cycle values (Cts), and melting curves were determined using the LightCycler 480 Software version 1.5 (Roche). ..

    Blocking Assay:

    Article Title: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes
    Article Snippet: Samples were diluted 1:5 within nuclease-free water (Gibco) and used alongside respective primers and LightCycler 480 SYBR Green I Master Kit (Roche). .. RT-qPCR reactions were performed in a LightCycler 480 Instrument II 384-well block (Roche) and quantification cycle values (Cts), and melting curves were determined using the LightCycler 480 Software version 1.5 (Roche). ..

    Software:

    Article Title: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes
    Article Snippet: Samples were diluted 1:5 within nuclease-free water (Gibco) and used alongside respective primers and LightCycler 480 SYBR Green I Master Kit (Roche). .. RT-qPCR reactions were performed in a LightCycler 480 Instrument II 384-well block (Roche) and quantification cycle values (Cts), and melting curves were determined using the LightCycler 480 Software version 1.5 (Roche). ..

    Article Title: Loss‐of‐function genetic screen unveils synergistic efficacy of PARG inhibition with combined 5‐fluorouracil and irinotecan treatment in colorectal cancer
    Article Snippet: Relative gene expression levels were calculated using the 2^(−ΔΔCt) method, with gene expression normalised to the reference gene β‐actin (IDT) and cells transfected with the control vector used as reference samples to determine relative expression levels. .. Data were analysed using LightCycler 480 software version 1.5 (Roche) for threshold cycle (Ct) determination. ..

    Article Title: Engineering a Perfusion Bioreactor System for hiPSC‐Derived Progenitor Co‐Culture Capturing Microglial Features in CNS Development
    Article Snippet: The reactions were performed with LightCycler 480 Instrument II 384‐well block (Roche, Basel, Switzerland). .. Quantification cycle values (Cq's) and melting curves were determined using the LightCycler 480 Software version 1.5 (Roche, Basel, Switzerland). ..

    Article Title: Loss-of-function genetic screen unveils synergistic efficacy of PARG inhibition with combined 5-fluorouracil and irinotecan treatment in colorectal cancer.
    Article Snippet: Relative gene expression levels were calculated using the 2ˆ(−ΔΔCt) method, with gene expression normalised to the reference gene β-actin (IDT) and cells transfected with the control vector used as reference samples to determine relative expression levels. .. Data were analysed using LightCycler 480 software version 1.5 (Roche) for threshold cycle (Ct) determination. ..

    Article Title: Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of tetA, tetB , and tetO genes in poultry and associated environments
    Article Snippet: .. The optimization steps were performed using the following amplification protocol: initial incubation at 50 °C for 2 min, followed by 2 min at 95 °C, and 45 cycles at 95 °C for 10 s and 50 °C−60 °C for 40 s. A melting curve between 40 °C and 95 °C was determined by adding a dissociation step after the last amplification cycle at a temperature transition rate of 4.4 °C/s. qPCR data analysis was performed using LightCycler ® 480 software version 1.5 (Roche). ..

    Article Title: Crispr-Cas systems having destabilization domain
    Article Snippet: .. Data were analyzed using the LightCycler® 480 Software, Version 1.5 (Roche) by the ΔΔCt method: target gene Ct values (FAM dye) were normalized to GAPDH Ct values (VIC dye), and fold-changes in target gene expression were normalized to RFP-transfected experimental controls. ..

    Amplification:

    Article Title: Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of tetA, tetB , and tetO genes in poultry and associated environments
    Article Snippet: .. The optimization steps were performed using the following amplification protocol: initial incubation at 50 °C for 2 min, followed by 2 min at 95 °C, and 45 cycles at 95 °C for 10 s and 50 °C−60 °C for 40 s. A melting curve between 40 °C and 95 °C was determined by adding a dissociation step after the last amplification cycle at a temperature transition rate of 4.4 °C/s. qPCR data analysis was performed using LightCycler ® 480 software version 1.5 (Roche). ..

    Incubation:

    Article Title: Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of tetA, tetB , and tetO genes in poultry and associated environments
    Article Snippet: .. The optimization steps were performed using the following amplification protocol: initial incubation at 50 °C for 2 min, followed by 2 min at 95 °C, and 45 cycles at 95 °C for 10 s and 50 °C−60 °C for 40 s. A melting curve between 40 °C and 95 °C was determined by adding a dissociation step after the last amplification cycle at a temperature transition rate of 4.4 °C/s. qPCR data analysis was performed using LightCycler ® 480 software version 1.5 (Roche). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of tetA, tetB , and tetO genes in poultry and associated environments
    Article Snippet: .. The optimization steps were performed using the following amplification protocol: initial incubation at 50 °C for 2 min, followed by 2 min at 95 °C, and 45 cycles at 95 °C for 10 s and 50 °C−60 °C for 40 s. A melting curve between 40 °C and 95 °C was determined by adding a dissociation step after the last amplification cycle at a temperature transition rate of 4.4 °C/s. qPCR data analysis was performed using LightCycler ® 480 software version 1.5 (Roche). ..

    Targeted Gene Expression:

    Article Title: Crispr-Cas systems having destabilization domain
    Article Snippet: .. Data were analyzed using the LightCycler® 480 Software, Version 1.5 (Roche) by the ΔΔCt method: target gene Ct values (FAM dye) were normalized to GAPDH Ct values (VIC dye), and fold-changes in target gene expression were normalized to RFP-transfected experimental controls. ..



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